Survey Prevalence Beta lactamase Enzyme in Bacterial Isolated of Hospital Environment (AZZAHRA Hospital–Isfahan-Iran)
Shilla Jalalpoor Teacher of Microbiology,
Islamic Azad University Shahreza Branch,
Membership of Young Researchers Club.
Islamic Republic of Iran
Abstract:
Introduction and Objectives:
β-lactame antibiotics (Penicillin-Cephalosporin) have selective poisoning and effective to more Bacteria then are very important in cure of diseases. b-lactamase is virulence agent and causes resistance to these antibiotics. According rule of hospital surface Bacteria in infection chain, transmission of β–lactamase producing Bacteria in patients, final result to β-lactame antibiotics resistance Bacteria.
Material and Methods:
The research were laboratory and performed in 2005/2007 years in AZZAHRA hospital in Isfahan, According to statistical formula selected 194 sample. Samples collected with swab in TSB from low and high contact surface. Identification Bacteria, were performed with microbiological methods and for determine β–lactamase production, use Acidimetric method.
Results:
From 194 study samples 70/35% product β–lactamase, respectively 82/70% Staphylococcus spp.,80/35% Enterobacteriaceae, 68/40% Bacillus spp. and 50% Pseudomonas.
Conclusion:
High spread β–lactamase in this Bactrian represent high spread Bactrian in surface hospital that due to facility transmission of β–lactamase genes in Bacteria. We offer despite of increase quality disinfectant agents, prevention transmission Bacteria to hospital, with controlling frequency people.
